Laboratory of translational Cellular and Molecular biomedicine

Laboratory of translational Cellular and Molecular biomedicine is the interdisciplinary research Laboratory, combining experimental approaches of cell and molecular biology, immunology, protein biochemistry, synthetic chemistry, clinical research, and advanced microscopy techniques. The research activities of the Laboratory are based on the concept of solving relevant scientific problems in areas of biomedicine and translation of basic scientific achievements in order to develop innovative preventive measures, early detection and effective personalized therapy for the most common and life-threatening diseases (cancer, cardiovascular disease and diabetes). It also develops innovative chemical and biological technologies for regenerative medicine.

Recently developed diagnostic markers and methods of their detection will be implemented into clinical practice for early diagnosis of malignant neoplasms and cardiovascular disease, for determination of sensitivity of cancer patients to existing schemas of therapy. Innovative therapeutic approaches will be used in personalized therapies of malignant neoplasms and cardiovascular diseases.

Main areas of research

For the first time we examine general patterns in the development of inflammation process in various types of diseases in the Laboratory. We will determine the highly specific markers that indicate the type innate immune activation in a particular pathology and in a particular patient. The most important goal is the research of new molecular and cellular processes that lead to the development of chronic subclinical inflammation. This research will be conducted on unique cellular model systems with the use of the most modern methods of systems biology and confocal microscopy. Long-term goal for the Laboratory for the next ten years is not only diagnosis of pathologic programming of innate immunity reactions and establishment of the fundamental molecular mechanisms of development of pathology. We are also aimed at using this data for optimization of existing therapeutic approaches and development of innovative immunomodulating therapy. In order to develop innovative therapy for chronic inflammation of different nature, we will conduct research in two areas:

  • Production of recombinant biologically active immunomodulatory proteins;
  • Development of a system for producing anti-inflammatory primary human macrophages in a form of a personalized cellular therapy drug.

Projects

  • To carry out fundamental research in areas of molecular and cell immunopathological mechanisms of the origin and development of the most dangerous diseases like cancer (breast cancer, lung cancer, stomach cancer, colon cancer, cancer of the brain) and cardiovascular disease of diabetic or infectious nature (myocarditis, heart failure, atherosclerosis, leading to heart attack and cerebral accident);
  • To develop highly sensitive and innovative methods for early diagnosis of immune pathologies leading to cancer and cardiovascular disease;
  • To develop innovative implant coatings with anti-inflammatory properties, and
  • To develop innovative therapeutic approaches based on recombinant purified proteins with antitumor and immunomodulatory effects, viral vectors (gene therapy) and nanoparticles.

Results

We conducted a large-scale research using genomic technologies and systems biology approaches to analyze expression profile of different subpopulations of human macrophages in cultural conditions. We found that each individual stimulus leads to highly-specific profiles of expression of molecular markers. We came to conclusion that the molecular phenotype of macrophages can be used as a diagnostic marker of subclinical inflammation and specific detection of type 2 inflammation. It was shown that macrophages have high-degree diversity and functional plasticity, which makes them the most promising cellular targets for the personalized therapy. We carried out a number of studies of epigenetic markers in the extracellular DNA associated with lung cancer and evaluation of diagnostic and prognostic value of these markers.

Actual tasks

We plan to develop on the basis of the spectrum of highly specific markers a test system for prognosis and selection of the optimal therapy for patients with heart failure and myocarditis depending on the presence of the viral pathogen.

We are going to work on a production system of drugs with biologically active recombinant proteins. We will also develop a fundamentally new approach to antitumor therapy of breast cancer, gastric cancer, and colon cancer. Target cells and molecular mechanisms of action of therapeutic proteins products will be defined.

We will develop a unique approach to the production of anti-inflammatory macrophages as a cell-therapeutic agent for the purpose of personalized treatment of patients with cancer of different localizations and patients with heart failure. These antibodies and recombinant proteins will serve as the basis for the creation of innovative, personalized therapeutic approaches to the treatment of cancerous and cardiovascular diseases.

The world's first large-scale study will be conducted to determine the fundamental differences of types of chronic inflammation, leading to the progress of the most life-threatening diseases of the two different types: cancer and cardiovascular.

Multidisciplinary approach will allow conducting system analysis of cellular functions, molecular processes and functional protein complexes, and identify new mechanisms of violations of differentiation and functioning of cells of innate immunity. This multidisciplinary approach is based on the most modern genomic, systems biological, molecular and cellular technologies, immunological methods, confocal microscopy, and unique biobank of clinical samples.

Faculty

Yulia G. Kzhyshkowska
Doctor of Biology
Head of the Laboratory for Translational Cell and Molecular Biomedicine TSU
Medical Centre Mannheim, University of Heidelberg, Germany
julia.kzhyshkowska@googlemail.com
 
Irina A. Kurzina
Doctor of Science (Physics and Mathematics)
Deputy head of the Laboratory for Translational Cell and Molecular Biomedicine TSU
Head of the Laboratory for Catalitic Research TSU
 
Nadezhda V. Cherdyntseva
Doctor of Biology
Leading researcher of the Laboratory
Deputy director for science of the Tomsk Саnсеr Rеsеаrсh Institute
 
Marina V. Zavyalova
Doctor of Medicine
Leading researcher of the Laboratory
Head of the Department for Pathological Anatomy in the Siberian State Medical University (Tomsk)
Researcher in the Tomsk Саnсеr Rеsеаrсh Institute, high level certificate physician
 
Evgeniya V. Kaygorodova
Doctor of Medicine
Leading researcher of the Laboratory
Researcher in the Tomsk Саnсеr Rеsеаrсh Institute, high level certificate physician, professor of the Siberian State Medical University (Tomsk)
 
Nikolay V. Litvyakov
Cand. Sc. (Biology)
Senior researcher of the Laboratory
Leading researcher in the Tomsk Саnсеr Rеsеаrсh Institute
 
Evgeniy V. Denisov
Cand. Sc. (Biology)
Senior researcher of the Laboratory
Leading researcher in the Tomsk Саnсеr Rеsеаrсh Institute
 
Natalia N. Babyshkina
Cand. Sc. (Medicine)
Senior researcher of the Laboratory
Leading researcher in the Tomsk Саnсеr Rеsеаrсh Institute
 
Evgeniya S. Grigorieva
Cand. Sc. (Medicine)
Researcher of the Laboratory
Researcher in the Tomsk Саnсеr Rеsеаrсh Institute
 
Matvey M. Tsyganov
Early Career Researcher of the Laboratory
 
Tatyana S. Gerashchenko
Early Career Researcher of the Laboratory
 
Mikhail A. Buldakov

Cand. Sc. (Biology)
Senior researcher of the Laboratory
Senior researcher in the Tomsk Саnсеr Rеsеаrсh Institute

 
Yuliya V. Rogovskaya
Cand. Sc. (Medicine)
Senior Researcher of the Laboratory
Head of the Department for Pathologoanatomy in Research Institute for Сardiology (Tomsk)
 
Roman E. Batalov
Cand. Sc. (Medicine)
Senior Researcher of the Laboratory
Senior Researcher in Research Institute for Сardiology (Tomsk)
 
Tatiana E. Suslova
Cand. Sc. (Medicine)
Senior Researcher of the Laboratory
Senior Researcher in Research Institute for Сardiology (Tomsk)
 
Victor V. Volkomorov
Early Career Researcher of the Laboratory
Researcher in the Tomsk Саnсеr Rеsеаrсh Institute
 
Darya Kokova
Early Career Researcher of the Laboratory
 
Lyudmila Rasskazova
Early Career Researcher of the Laboratory
 
Natalia B. Dementyeva
Early Career Researcher of the Laboratory
 
Elena G. Shapovalova
Early Career Researcher of the Laboratory

Master's Degree Student

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